109 - Box Hill - Port Melbourne
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To Box Hill
Trams commence at Waterfront Place (Port Melbourne) and run via the Light Rail, Clarendon St, Spencer St, Collins St (City), Macarthur St, St Vincents Plaza, Victoria Pde, Victoria St, Barkers Rd, High St, Cotham Rd and Whitehorse Rd to the terminus near Market St (Box Hill).
To Port Melbourne
Trams commence in Whitehorse Road near Market St (Box Hill) and run via Whitehorse Rd, Cotham Rd, High St, Barkers Rd, Victoria St, Victoria Pde, St Vincents Plaza, Macarthur St, Collins St (City), Spencer St, Clarendon St and the Light Rail to Waterfront Place (Port Melbourne).